Estudo comparativo entre as provas de imunodifusao em placa e em lamina na deteccao de anticorpos contra o virus da leucose enzootica bovina []. Contagem linfocitaria e anticorpos contra o virus da leucose enzootica bovina em rebanhos do Rio de Janeiro []. Ferreira, M.I. Romero, C.H. Rowe, C.A. Ocorrência do vírus da leucose enzoótica dos bovinos (BLV) e de anticorpos contra herpesvírus bovino tipo-1 (BoHV-1) e vírus da diarreia viral bovina (BVDV) .

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Once infected, the bovine remains as a virus carrier for life and such state correlates with the specific antibody detectable level.

For the challenge test, the sensitivity was The bottom line appears to be that transmission relies primarily on the transfer of infected lymphocytes from one animal to the next and that BLV positive animals with lymphocytosis are more likely to provide a source for infection. In more recent years, different ELISAs have been used in eradication programs and there are several commercial kits available to detect antibodies against the principal viral proteins gp51 and p24 Only some animals later develop a B-cell leukemia called enzootic bovine leukosis.

Bovia optical density average values for each serum were passed onto forms and processed using Epilnfo version 5.

Recebido em 19 de abril de Aceito em 13 de abril de After artificial infection of sheep most animals succumb to leucos. Testing and removing positive animals from the herd is one method of control. Results showed that The protein constitution of the antigen was analyzed by Western Blot following the technique previously described by Gonzalez et al.

Natural infection of animals other than cattle and buffalo are rare, although many animals are susceptible to artificial infection. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution.


This information is passed onto a 2 x 2 contingency table, which constitutes the standard for presenting the results to calculate sensitivity, specificity, prevalence and predictive values The Leucokit-La Plata is appropriate to apply to the commercialization of live bovines to export, bovine selection for hemo-vaccines and the implementation of control and eradication programmes. Services on Demand Journal.

All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. PCR, a recent molecular biological tool, remains labour intensive and expensive to perform which limit the use to large commercial or research oriented diagnostic laboratories The Youden’s Index J 7 was 0.

But it is not known whether this naturally occurring rabbit disease is linked to BLV infection. Molecular diagnostic procedures PCR should not necessarily replace serological procedures, like ELISA, currently in use that had been proben to be cost-effective, rapid, sensitive and reliable and which are recognised as official test for commerce and trade purposes. In herds where the disease is widespread, it is important to limit spread by avoiding contact with blood between animals.

The interpretation of the Kappa value in this case has leucsoe since it was applied to two different serological tests ID and indirect ELISA and Kappa implicates that challenged tests are equivalent It is possible that a natural virus reservoir exists in the water buffalo.

Artigos sem imagem tanto localmente quanto no Wikidata! On the other hand, Souza I ; A. Applying Kappa and Jouden’s Index J coefficients an almost perfect concordance leeucose obtained between both kits.

Once the bovine is infected, it remains as a virus carrier for life and such state is correlated with a specific antibody detectable level. Flow cytometry detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin-V. In this case the value was 0.

Apoptosis of CD5+ cells and lymphocyte proliferation in bovine leukemia virus-infected dairy cows

That value showed the best correspondence between our challenge test Leucokit-La Plata and the gold standard test ID. The Apparent Prevalence was Saunders Elsevier,p. Bovine Leukosis Virus EBLV is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle.


The correspondence between the two tests Fig. So for artificial infection infected cells are used leucosf the more stable and even heat resistant DNA. Also in this case the Youden’s Index J showed a high concordance 0. In enzoktica cases and as pointed out by Kozaczynska 15sample analyses should be repeated, keeping the animals as no conclusive until the definite confirmation of the result is obtained.

Reduced cell turnover in bovine leukemia virus-infected, persistently lymphocytotic cattle. A rapid and sensitive diagnosis of bovine leukaemia virus enzooica using the nested shuttle polymerase chain reaction. CD5 is associated from the B-cell receptor in B cells from bovine leukemia virus-infected, persistently lymphocytotic cattle: But neither leukemia nor other signs of infection could be detected.

Leucose enzoótica bovina

Like the North American states, those of the Eastern block in Europe did not try to get rid of the virus. In general BLV causes only a benign mononucleosis leuose disease enzoorica cattle. Frontiers in Biosciencev. These efforts are based on the application of tests such as immunodiffusion IDenzime linked immunosorbent assay ELISAwestern blot WB and polymerase chain reaction PCR ,13,15,20,24,25following the separation or removal of the virus-carrier animals.

Emphasis on cell turnover in two hosts infected by bovine leukemia virus; a rationale for host susceptibility to disease.